Effect of hydrogen sulfide (H2S) on the growth and development of tobacco seedlings in absence of stress.

BACKGROUND: Hydrogen sulfide (H2S) is a novel signaling molecule involved in the growth and development of plants and their response to stress. However, the involvement of H2S in promoting the growth and development of tobacco plants is still unclear. RESULTS: In this study, we explored the effect of pre-soaking or irrigating the roots of tobacco plants with 0.0, 2.0, 4.0, 6.0, and 8.0 mM of sodium hydrosulfide (NaHS) on endogenous H2S production, antioxidant enzymatic and cysteine desulfhydrase activities, seed germination, agronomic traits, photosynthetic pigments contents, and root vigor. The results revealed that exogenous NaHS treatment could significantly promote endogenous H2S production by inducing gene expression of D/L-CD and the activities of D/L-CD enzymes. Additionally, a significant increase in the agronomic traits and the contents of photosynthetic pigments, and no significant difference in carotenoid content among tobacco plants treated with 0.0 to 8.0 mM of NaHS was observed. Additionally, a significant increase in the germination speed, dry weight, and vigor of tobacco seeds, whereas no significant effect on the percentage of seed germination was observed on NaHS treatment. Furthermore, NaHS treatment could significantly increase the activity of superoxide dismutase (SOD) and peroxidase (POD) enzymes, which reduces damage due to oxidative stress by maintaining reactive oxygen species homeostasis. CONCLUSIONS: These results would aid in enhancing our understanding of the involvement of H2S, a novel signaling molecule to promote the growth and development of tobacco plants.

The inhibiting effects of organic acids on arsenic immobilization by ferrihydrite: Gallic acid as an example.

Organic acids usually compete the immobilization of As by iron (hydro)oxides, but their oxidizing effects are ignored. Therefore, the gallic acid (GA) with strong redox activity was chosen to investigate the influence of arsenite [As(III)] oxidation on As immobilization by ferrihydrite. Our results found that the As amount adsorbed on ferrihydrite decreased with the pH rising from 5 to 9 in the presence of GA, and the adsorption amount (28.8 g kg-1) at pH 9 was 45.1% lower than that in the absence of GA. Meanwhile, the As adsorption amounts in treatments of GA addition before As (Fh-GA-As(III)) were significantly lower than that in their corresponding simultaneous addition (Fh-As(III)/GA). The proportions of As(V)/Astotal on ferrihydrite and in equilibrium suspension were increased as the pH increased in the presence of GA, and the highest oxidation efficiency of As(III) by GA at pH 9 was 90.3%, which was mainly due to the contribution of hydrogen peroxide (H2O2, 52.6%) and semiquinone radicals (SQ-, 27.1%). In general, the oxidation and competition adsorption of As by GA inhibited the As immobilization by ferrihydrite, and the oxidation of As(III) by GA was strongly dependent on pH, while H2O2 and SQ- were demonstrated as the main oxidant at pH 9.

Effect of P/As molar ratio in soil porewater on competitive uptake of As and P in As sensitive and tolerant rice genotypes.

Phosphorus (P) can affect the bioavailability and mobility of arsenic (As) in paddy soil-plant system, but it is not clear how different forms of phosphorus fertilizers affect P/As molar ratio in soil and how the ratio in turn affects the competitive uptake of P and As in two genotypes. Different P fertilizers, i.e., calcium-magnesium phosphate (CMP), superphosphate (SP) and potassium phosphate monobasic (PPM), were used to investigate the difference in competitive uptake between As sensitive (IIY3301) and As tolerant (SY9519) rice genotypes. Our results indicated that the contents of total As in brown rice of PPM and SP treatments (II-PPM and II-SP) were 15.4% and 26.9% lower than that of CMP treatment (II-CMP) for IIY3301 genotype, but their P contents were 27.0% and 17.8% higher than that of II-CMP treatment. However, the As content in brown rice showed no significant difference between PPM and CMP treatments for SY9519 genotype (S-PPM and S-CMP). The net As accumulation in shoots of II-PPM during the tillering stage was significantly lower than that of II-CMP, but the difference of net As accumulation between S-PPM and S-CMP was not significant. The As translocation factor in II-PPM and II-SP were 16.7% and 22.2% lower than that in II-CMP, but the difference of As translocation factor between S-PPM and S-CMP was not significant. In addition, the contents of total As in porewater showed no significant difference between PPM and CMP. Conversely, the P/As molar ratio in porewater of PPM during tillering stage was 10.9% higher than that of CMP. In summary, PPM led to a higher P/As molar ratio in porewater, which promoted the competitive uptake of As and P by IIY3301 genotype; and the competitive uptake of As and P was more likely to occur in As sensitive rice genotype than in As tolerant rice genotype.